The presence of an embryo affects day 14 uterine transcriptome depending on the nutritional status in sheep. b. Immune system and uterine remodeling

Transcriptomics and bioinformatics were used to investigate the potential interactions of undernutrition presence conceptus at time maternal recognition pregnancy on uterine immune system remodeling. Adult Rasa Aragonesa ewes allocated one two planes nutrition for 28 days: maintenance energy intake (control; 5 cyclic, 6 pregnant ewes) providing 7.8 MJ metabolisable 0.5 (undernourished; 7 3.9 per ewe. Uterine gene expression was measured using Agilent 15 K Sheep Microarray chip day 14 estrus or pregnancy. Functional analyses performed PANTHER (Protein ANalysis THrough Evolutionary Relationships) Classification System. Pregnancy affected 18 genes in both control undernourished ewes, underscoring relevance embryo-maternal interactions. Immune evidenced by classical interferon stimulated activated -in a lesser extent-in vs cyclic ewes. Genes involved remodeling such as protein metabolism also upregulated with an embryo However, relevant adaptation uterus differentially expressed between groups. Undernutrition alone led overall weak activation pathways Data revealed that cellular adaptations are dependent nutritional status.